#SYMPHYTUM OFFICINALE TINCTURE FREE#

Our findings suggest that homoeopathic dose (specially mother tincture) of Symphytum officinale has the potential to enhance osteogenesis.īone healing Gene expression Homoeopathy Mesenchymal stem cells Osteoblasts Osteogenesis Symphytum officinale Traditional medicine Asia & Oceania.Ĭopyright © 2019 Elsevier B.V. Interestingly, gene expression analysis for Runx-2 (n = 10), Osteopontin (n = 10), Osteocalcin (n = 10) and alkaline phosphatase activity (n = 8) also showed increased osteogenesis with the addition of Symphytum officinale to BDM, specially mother tincture. The efficiency of osteogenic differentiation (2 weeks) for different doses of Symphytum officinale was determined by flow cytometry (n = 10) for osteoblast marker, Osteocalcin, and most doses of Symphytum officinale enhanced osteogenesis. Fresh organic leaf tincture - 1:5 25 - 500ml, 1000ml. Shop > Tinctures and fluid extracts > Symphytum officinale NB.

#SYMPHYTUM OFFICINALE TINCTURE FREE#

MTT assay showed that the selected doses of SO did not induce any cytotoxicity in MSCs (24 hours). FREE MAINLAND SHIPPING ON ORDERS OVER £150 excluding VAT. Gene expression analyses for osteoblast markers (Runx-2, Osteopontin and Osteocalcin) were evaluated in differentiated osteoblasts using qPCR.įlow cytometry (CD90, CD105) detected MSCs isolated from bone marrow (93-98%). Different homoeopathic doses of SO (MT, 3C, 6C, 12C and 30C) were added to the basic differentiation medium (BDM) and efficiency of MSCs differentiating into osteoblasts were measured by evaluating expression of Osteocalcin using flow cytometry, and alkaline phosphatase activity using ELISA. Osteogenic differentiation was induced in MSCs with β-glycerophosphate, ascorbic acid and dexamethasone over 2 weeks. Cytotoxicity of SO was evaluated by MTT assay. 97 (7. Even though this plant contains poisonous chemicals called pyrrolizidine alkaloids (PAs), some people use the leaf, root, and root-like. MSCs were isolated, expanded and characterized by flow cytometry (CD90, CD105). Comfrey Leaf Alcohol-Free Liquid Extract, Organic Comfrey (Symphytum Officinale) Dried Leaf Tincture Supplement (4 FL OZ) 21 29.97 29.

This study aimed to determine if homoeopathic doses of SO could augment osteogenesis in MSCs as they differentiate into osteoblasts in vitro.īone marrow samples were obtained from patients who underwent bone grafting procedures (n = 15). It reduces the swelling and pain and promotes union of bones. Comfrey (Symphytum officinale) is a perennial herb with large, hairy leaves that bears small bell-shaped flowers of various colors.Comfrey has been used since ancient times: The ancient Romans and Greeks recommended comfrey for wound healing and broken bones as well as inflammatory and traumatic lesions of muscles and joints. Bones: It hastens the healing of fractures. Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, believed to accelerate bone healing. SBL Symphytum Officinale Mother Tincture Q. Mesenchymal stem cells (MSCs) are multipotent stem cells possessing regenerative potential.

Symphytum officinale tincture